TCLP (Toxicity Characteristic Leaching Procedure)
TCLP or Toxicity Characteristic Leaching Procedure is carried out to determine both organic and inorganic analyte mobility in liquid waste, solid waste, and multiphase. It is usually used to determine whether the waste includes in toxic definition based on the category of US-EPA.
The analysis of TCLP simulates the condition of Final Disposal Site (TPA). Over the time, the water and other liquids that are derived from the waste will permeate through Final Disposal Site (TPA). The liquid that permeate often reacts with solid waste and can create a risk of public health and environment due to the contaminant. The analysis of TLCP will determine the identified toxic contaminant (based on Environmental Protection Agency/EPA) in the form of lye and its concentration.
LC50 (Median Lethal Concentration)
LC50 (Median Lethal Concentration) is a biological toxicity test using test animals to know the concentration, which causes the death of test animals as much as 50%. The observation time is commonly 96 hours and the test animals used refer to US-EPA. Statistical test is performed to know the value of LC-50.
There are two phases in the research, namely: preliminary test (to determine the critical threshold concentration, i.e. the concentration that causes the largest death up to 50% and the smallest death up to 50%) and advanced test (after the critical threshold is identified, then the acute concentration is determined based on the series of concentration logarithm).